A novel universal multiplex PCR improves detection of AZFc Y-chromosome microdeletions
نویسندگان
چکیده
منابع مشابه
Multiplex PCR Screening of Y Chromosome Microdeletions in Azoospermic Patients
Objective To develop a multiplex PCR protocol for routine screening of microdeletions on the Y chromosome Methods Five multiplex sets were established and Y chromosome microdeletions screening were carried out in 26 azoospermic men who undertook ICSI and 30 azoospermic men who undertook testicular biopsy. Results In 56 azoospermic men, 5 patients were found with AZFc/DAZ microdeletions, 2 patie...
متن کاملDetection of sperm in men with Y chromosome microdeletions of the AZFa, AZFb and AZFc regions.
BACKGROUND Y chromosome microdeletions are associated with severe male factor infertility. In this study, the success rate of testicular sperm retrieval was determined for men with deletions of AZF regions a, b or c. METHODS AZF deletions were detected by PCR of 30 sequence-tagged sites within Yq emphasizing the AZFa, b and c regions. Semen analysis and diagnostic testis biopsy or testicular ...
متن کاملScreening for Y-chromosome microdeletions in infertile Indian males: utility of simplified multiplex PCR.
BACKGROUND & OBJECTIVE Analysis of the microdeletions in the azoospermia factor (AZF) region of Y chromosome by PCR is an important screening tool in the work-up of infertile males opting for assisted reproductive techniques. In the present study, the Y chromosome microdeletions were analyzed by PCR using primers corresponding to 16 sequence tagged sites (STS) and three genes of the AZF region ...
متن کاملMultiplex PCR Screening of Y-chromosome microdeletions in azoospermic ICSI candidate men
BACKGROUND It has been hypothesized that Y-q microdeletion can account for significant proportion of infertility in men. There are three nonoverlapping regions referred to as the "azoozpermia factors" AZFa, AZFb, and AZFc from proximal to distal part of Y-q. These have been defined as spermatogenesis loci, this region deletions have been shown to be involved in male azoospermic or severe oligoo...
متن کاملDetection of Y Chromosome Microdeletions and Hormonal Profile Analysis of Infertile Men undergoing Assisted Reproductive Technologies
Objective Y chromosome deletions (YCDs) in azoospermia factor (AZF) region are associated with abnormal spermatogenesis and may lead to azoospermia or severe oligozoospermia. Assisted reproductive technologies (ART) by intracytoplasmic sperm injection (ICSI) and testicular sperm extraction (TESE) are commonly required for infertility management of patients carrying YCDs. The aim of this study w...
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ژورنال
عنوان ژورنال: Journal of Assisted Reproduction and Genetics
سال: 2014
ISSN: 1058-0468,1573-7330
DOI: 10.1007/s10815-014-0204-5